An immunohistochemical study of bFGF and COX2 after human brain contusion / 中国法医学杂志

ABSTRACT

Objective Study the changes of bFGF, COX2 after human severe brain injury and find an effective method for estimating time interval after brain injury.Method 35 brain tissue samples of severe brain injury were examined using immunohistochemical staining and image analysis technique to evaluate the expression of bFGF and COX2.Results Maximal bFGF expression was found at 0h after brain contusion. The intensity of bFGF staining decreased remarkably 12h after brain contusion and descended gradually to the minimum on the 11th day. Expression of COX2 positive cells increased significantly 12h after brain contusion and reached the maximum level one day after brain contusion. Then the expression decreased gradually from the 2nd day to almost aero on the 11th day.Conclusion The changes of bFGF and COX2 were regular along with various survival time after brain contusion so that bFGF and COX2 immunohistochemical staining can be used as a referential data for estimating time interval after human brain contusion.