An experimental study on inhibition of apoptosis by HBx to human hepatic carcinoma cells in a nude mouse model / 中国普通外科杂志

ABSTRACT

Objective To investigate the effects of HBx on proliferation and apoptosis of human hepatocellular carcinoma cell line HepGz in vitro and in vivo. Methods The expression plasmids of pHA-HBx encoding full length of HBx gene was transfected into HepG2 cells with Lipofectamine 2000. The proliferation activity of transformed cells was measured by calculating the growth curve,population doubling time and by the 3H-TdR incorporation rate assay. The nude mouse model of HepG2 expressing HBX gene was established and the apoptosis rate of tumor cells was detected with TUNEL assay. Results RT-PCR indicated that the HBx gene was integrated into the genome DNA of HepG2 cells and transcripted. The growth curve and population doubling time manifested that the proliferation activity of transformed cells was much higher than that of control group cells. The apoptosis rate of tumor cells expressing HBx gene was much lower than that of control group cells. ConclusionHBx facilitates the proliferation activity of hepatoma cells in vitro and in vivo and inhibits the apoptosis of hepatoma cells induced by adriamycin in nude mice.