Determination of Diclofenac Sodium in Huamn Plasma by HPLC / 中国药房
China Pharmacy
; (12)2001.
Article
em Zh
| WPRIM
| ID: wpr-521746
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WPRO
ABSTRACT
OBJECTIVE:To establish a HPLC procedure to determine diclofenac sodium in human plasma.METHODS:The plasma sample was deproteinized by adding acetonitrile,A Hypersil-BDS(250?4.6mm,5?m)column with a mobile phase consisting of methanol-water-10%phosphate acid(60∶40∶1)was used,the detecting wavelength was276nm,column tem?perature was35℃.RESULTS:The linear range was20.50~2050.0ng/ml(r=0.9998,n=5),the detective limit was10ng/ml.The recovery was99.47%~105.4%with the within-day RSD from3.77%to5.81%(n=5),and the between-day RSD from4.93to8.96%(n=5)at three different concentrations.CONCLUSION:The method is simple,rapid and suitable for the pharmacokinetic study of diclofenac sodium.
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Índice:
WPRIM
Idioma:
Zh
Revista:
China Pharmacy
Ano de publicação:
2001
Tipo de documento:
Article