The opening of neuronal mitochondria permeability transition pore affects respiratory function during thrombotic cerebral ischemia in tree shrews / 中国病理生理杂志

ABSTRACT

AIM: The present study explored those changes in brain mitochondrial respiration in different times after thrombotic cerebral ischemia induced by photochemical reaction in primate's animal tree shrew and o bserved the effects of platelet-activating factor (PAF) receptor antagonist gin kgolide B (GB) and immunosuppressor cyclosporin A (CsA) on neuronal mitochondria l respiration and mitochondrial permeability transition pore(MPT) in twenty-four hours after occlusion and showed their neuronprotective mechanism and expatiate the relationship between mitochondrial respiration and MPT. METHODS: The focal thrombotic cerebral ischemia was formed by photochemistry-induce d technology in tree shrews. At 4,24,72 h after focal cerebral ischemia, the n euronal mitochondria were centrifuged. Clark oxygen electrode was used to measur e the changes in neuronal mitochondrial respiration.Pretreatment for experiment al animals with GB and CsA at 6h after occlusion, we centrifuged the mitochondri a and measured the changes in neuronal mitochondrial respiration at 24 h after o cclusion. In addition, experiments were performed in a flurometer by measuring CaCl 2 100 ?mol/L induced centrifuged mitochondrial swelling and GB or CsA were added at the same time. RESULTS: All of respiration state Ⅲ, RCR and P/O decreased after cerebral ischemia. There were significant difference s between every ischemic group and sham, especially at 24 h (P