Cpn60 deficiency may play a role in the intrapancreatic enzyme activation in acute pancreatitis / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1986.
Artigo
em Chinês
| WPRIM
| ID: wpr-522153
ABSTRACT
AIM:
To investigate the change of Cpn60 content,the alterations of pancreatic enzymes and lysosome,in order to better understand the mechanism of intrapancreatic enzyme activation in acute pancreatitis(AP).METHODS:
The AP model was replicated by retrograde infusion of 4% sodium-deoxycholate in the choledocus of SD rats. The levels of amylase in plasma and TNF-? in pancreatic tissue were measured by biochemical technique at 5 h and 10 h after AP induction. The content of Cpn60 and pancreatic enzymes in different compartments of the acinar cells were tested by quantitative protein A-gold immunocytochemistry technique. The change of lysosome in the acinar cells was observed under the electronic microscope.RESULTS:
After AP was induced,the levels of amylase in the plasma and TNF-? in the pancreatic tissue increased significantly. Lysosomes with different forms were found inside the acinar cells,and some of them located in the Golgi apparatus. Cpn60 content decreased,which was accompanied by an increase of lipase or chymotrypsinogen content in the pancreatic secretory pathway.CONCLUSION:
In the pancreatic acinar cells of AP rats,Cpn60 content decreased,suggesting an insufficient chaperone capacity,and combining with the change of lysosome both in its amount and location,which may take part in the intrapancreatic enzyme activation and the development of AP.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Pathophysiology
Ano de publicação:
1986
Tipo de documento:
Artigo
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