Study on Microsatellite DNA Loss of Heterozygosity in p16 Gene of Psoriatic Keratinocytes / 中华皮肤科杂志
Chinese Journal of Dermatology
; (12)1995.
Article
em Zh
| WPRIM
| ID: wpr-522208
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WPRO
ABSTRACT
Objectives To detect the loss of heterozygosity(LOH)frequencies of microsatellite loci D9S171and D9S1604in p16gene of psoriatic keratinocytes,and to study the correlation between mi-crosatellite LOH of p16gene and the development of psoriasis.Methods By the use of polymerase chain reaction(PCR)-denaturing polyacrylamide gel electrophoresis-silver staining,LOH was detected with23sam-ples of keratinocytes from psoriatic lesions and non-lesion skin.Results LOH was identified at loci D9S171and D9S1604in5and10out of23keratinocyte samples from LOH-exhibited psoriatic lesions,and in2and3of keratinocyte samples from psoriatic non-lesion skin,respectively.The frequency of LOH at D9S1604was significantly higher in psoriatic lesion samples than that in psoriatic non-lesion skin(P
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Índice:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Dermatology
Ano de publicação:
1995
Tipo de documento:
Article