Effects of hydrogen peroxide on intracellular free calcium concentration in cultured neonatal rat cardiomyocytes and its antagonism by taurine / 中国病理生理杂志

ABSTRACT

AIM: To examine the effects of hydrogen peroxide on intracellular free calcium concentration([Ca~(2+)]i) in cardiomyocytes and its antagonism by taurine. METHODS: A cell culture model of neonatal rat cardiac myocytes was used. There were four groups, control group, hydrogen peroxide (H_2O_2) group, H_2O_2+taurine (simultaneously) group,and H_2O_2+taurine (in sequence) group. Confocal microscope was used with Fluo-3/AM as calcium indicator to detect changes of [Ca~(2+)]i immediately and 15 minutes after H_2O_2 intervention, respectively. RESULTS:The intracellular fluoresence intensity of singular cardiomyocyte in H_2O_2 group was significantly higher than the control group 15 minutes after intervention (P