Effect of adrenomedullin on L-type calcium currents and its signaling transduction process in guinea-pig ventricular myocytes / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)1986.
Article
em Zh
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| ID: wpr-522461
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ABSTRACT
AIM: To study the effect of adrenomedullin (ADM) on L-type calcium currents (I Ca,L) and its signaling transduction process in guinea-pig ventricular myocytes. METHODS: By using whole-cell patch clamp technique, the effect of ADM(1-100 nmol?L -1) on I Ca,Lmyocyte. Furthermore, the possible signaling transduction process of ADM on I Ca,L was investigated by observing the effects of ADM 22-52 (a specific ADM-receptor antagonist,100 nmol?L -1)+ADM(100 nmol?L -1), H-89 (a specific protein kinase A inhibitor, 10 ?mol?L -1) + ADM(100 nmol?L -1), PKC 19-36 (a specific protein kinase C inhibitor, 10 ?mol?L -1) + ADM(100 nmol?L -1) and PMA (a specific protein kinase C activator, 1 ?mol?L -1) on I Ca,L, respectively. RESULTS: ADM at the concentrations of 1-100 nmol?L -1 decreased I Ca,L in a dose-dependent manner (P
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WPRIM
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Zh
Revista:
Chinese Journal of Pathophysiology
Ano de publicação:
1986
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Article