5-aza-2-deoxycytidine inhibits the growth of bile duct cancer cell line in vivo and in vitro / 中国普通外科杂志

ABSTRACT

Objective To study the effects of 5-aza-2-deoxycytidine (ZdCyd), a methylation inhibitor, on the growth of bile duct cancer cell line QBC939 in vivo and in vitro. MethodMTT method and flow cytometry were used to detect the growth and apoptosis of QBC939 after being treated with different dosage of ZdCyd singly or in combination with other chemotherapy drugs. Nude mouse model was used to test the effect of ZdCyd. Results5-aza-2-deoxycytidine blocks cell cycle at G1 phase increasing apoptosis rate. These effects within certain extent are dose and time dependent.5-aza-2-deoxycytidine also coordinates with other chemotherapy drugs in the anti-tumor effects. In nude mouse model the tumor occurrence rate decreased in 5-aza-2-deoxycytidine pro-treated cells of QBC939, and ZdCyd also impedes the growth of transplanted tumor. Conclusion 5-aza-2-deoxycytidine inhibits the growth of QBC939 in vivo and in vitro by inducing cell apoptosis and enhances the antitumor effect of chemotherapy drugs.