A Study on Unilineage Differentiation of Umbilical Cord Blood CD_(34)~+ Cells Into Granulocytes, Erythrocytes or Platelets / 中国医师杂志

ABSTRACT

Objectives To establish culture system for unilineage differentiation of umbilical cord blood CD 34 + cells into granulocytes, erythrocytes or platelets. Methods After CD 34 + cells were separated by midiMACS using micro beads conjugated with anti-CD 34 monoclonal antibody, these cells were induced to specifically differentiate along granulocyte, erythrocyte or platelet by adding appropriate hematopoietic growth factors including SCF plus G-CSF, EPO or TPO. Then morphology, immunological phenotype and function analysis were performed to identify these induced cells. Results After induced unilineage differentiation, the percentages of CD 15 +, GPA + and CD 41 + cells were 81 17%, 95 35% and 77 82%, respectively. These induced cells acquired terminal cell's morphologies. The cells from the granulocytic culture had the ability to phagocytose Chinese ink and plate-like particles could aggregate under the action of thrombin. Conclusion The culture system for unilineage differentiation of CD 34 + cells into erythrocytes, granulocytes, or platelets was established, which was a basis for the research about cell treatment, gene regulation and exogenous gene expression in hemopoiesis.