Activation of caspase-3 during bacterial redox protein azurin -induced apoptosis in U2OS cells / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1999.
Artigo
em Chinês
| WPRIM
| ID: wpr-523302
ABSTRACT
AIM:
To study whether caspase-3,8 is activated during azurin-induced apoptosis in U2OS cells.METHODS:
AnnexinV /PI method was used to detect apoptosis. The changes of procaspase-3 were analyzed by Western blot, the changes of caspase-3 mRNA were detected by semi-quantitative RT-PCR, and caspase-3 relative activity was determined by colorimetric assay.RESULTS:
After U2OS cells were treated with 0, 25, 50, 100, 200, 500 mg/L azurin for 24 h, respectively, the level of procaspase-3 protein decreased and the level of caspase-3 mRNA increased as azurin concentration increased. When the cells were treated with 100 mg/L azurin for 6, 12, 24, 48 h , respectively, the caspase-3 activity began to rise from 6 h,reached the peak at 24 h,and was still higher than the control group at 48 h ( P
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Pathophysiology
Ano de publicação:
1999
Tipo de documento:
Artigo
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