Influence of Gentamycin Electrophysiological Properties on Murine Cochlea Spiral Ganglion Neurons / 听力学及言语疾病杂志

ABSTRACT

Objective To explore the influence of gentamycin on murine cochlea spiral ganglion neurons' electrophysiological properties and its significance.Methods Using whole-cell voltage clamp technique, we studied gentamycin's influence on the peak currents of the potassium and sodium ion channels on cell membranes of acutely dissociated murine spiral ganglion neurons,the relationship to gentamycin's concentration in extracellular fluid, and the currents' recovery after gentamycin being washed out.Results Gentamycin could inhibit voltage-dependent potassium channels, but it couldn't inhibit voltage-dependent sodium channels. Gentamycin's inhibitation on potassium currents had dose-dependence with gentamycin's concerntration in extracellular fluid and the currents recoverd incompletely after gentamycin being washed out.Conclusion This research explained the ototoxic mechanism of gentamycin through its action on keeping from spiral ganglion neurons' potassium ion channels from the electrophysiological aspect, and set a foundation for further research.