Recombinant PML adenovirus suppresses human gallbladder cancer cell growth by inducing apoptosis / 中国普通外科杂志

ABSTRACT

Objective To explore the molecular mechanism by which promyelocytric leuxemia (PML) suppresses human gallbladder cancer cell line (GBC-SD) growth.Methods GBC-SD cells were infected by green fluorescent protein recombinant adenovirus (Ad-GFP), GFP-positive cells were examined by microscopy.Cultured gallbladder carcinoma cells (GBC-SD) were infected with Ad-PML or Ad-control.Cell death was detected by DNA laddering and TUNNEL analysis.Cell cycle was analyzed by flow cytometer.Results An infection efficiency of 100% can be achieved at a concentration of 100 multiplicity of infection (MOI).The growth rate of the Ad-PML-infected GBC-SD cells was significantly inhibited.DNA laddering was detected at 72 h post-infection.The amount of apoptotic cells significantly increased in the Ad-PML-infected GBC-SD cells without evident alterations in cell cycle distribution.Conclusion PML suppresses growth of human gallbladder cancer cell line GBC-SD by inducing apoptosis.