Protective effects of Pinacidil on neurons in rat after anoxic/hypoglycemic damage / 中华围产医学杂志

ABSTRACT

Objective To investigate the protective effects of ATP-sensitive potassium channel (K (ATP)) opener (Pinacidil) on cultured cortical neurons in newborn rats after anoxic/hypoglycemic damage. Methods Neurons were exposed to anoxic/hypoglycemic condition for 4 h,8 h,16 h, after being cultured for 10 days. All cultured neurons were divided into 4 groups normal control group, model control group, Pinacidil (Pin) group and Pin plus Glibenclamide (Gli) group. The cell mortality, quantity of LDH and the percentage of neuron apoptosis in each group were determined. Results Pinacidil (10~(-6)mol/L ) reduced the percentage of cell death(4 h21.42?3.68 vs 14.83?2.94; 8 h36.58?6.14 vs 19.25?3.33; 16 h49.17?8.3 vs 28.64?6.4, P