Apoptosis of lens epithelial cells induced by elemene / 中国病理生理杂志

ABSTRACT

AIM: To investigate the effect of natural medicinal monomer elemene (Ele) on apoptosis of lens epithelial cells (LEC) and its the cellular and molecular mechanism in vitro. METHODS: The bovine LEC were cultured with Ele and used to observe the ultrastructure changes under transmission electron microscope and to detect DNA content and mitochondrial transmenbrane potential (??m) changes by flow cytometry. RESULTS: The typical morphological changes of LEC apoptosis in Ele group were observed under transmission electron microscope, such as chromatin condensation and aggregation at the nuclear periphery, and nuclear fragmentation as well. The DNA content of LEC in Ele group decreased and showed time-dependent. It was significant lower than that in control group (P