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Identify Polysaccharide in Holothuria atra Jeager by Spectroscopy / 中国药房
China Pharmacy ; (12)2005.
Artigo em Chinês | WPRIM | ID: wpr-525208
ABSTRACT

OBJECTIVE:

To establish the method to identify the polysaccharide element in Holothuria atra Jeager by spectroscopy.

METHODS:

The purity of polysaccharide in Holothuria atra Jeager was determined by electrophoretic method;the infrared absorption spectrum,ultraviolet absorption spectrum,carbon spectra and hydrogen spectra of NMR were identified in structure by infrared spectrophotometry,ultraviolet spectrophotometry and NMR spectrometry respectively.

RESULTS:

The gel band after electrophoresis took blue color and the results of spectrum determination accountable for each other.

CONCLUSION:

Uniformly constituent of polysaccharose was found in the Holothuria atra Jeager,which was an acidic polysaccharose with vitriolic esterfunction,without glucopeptide;?-configuration,?-glucopyranose was found and the intramolecular hydrogen bond was formed by hydroxide radical.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: China Pharmacy Ano de publicação: 2005 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: China Pharmacy Ano de publicação: 2005 Tipo de documento: Artigo