Polymorphic expression of UDP-glucuronosyltransferase UGT1A gene locus in human colorectal epithelium / 中国病理生理杂志

ABSTRACT

AIM: To analyze the polymorphic expression of UDP-glucuronosyltransferase UGT1A gene locus in human colorectal epithelium. METHODS: Colorectal tissue samples were obtained from 40 patients with colorectal cancer and 20 normal controls. The levels of UGT1A transcriptions were detected by RT-PCR, exon-1 specific RT-PCR. UGT1A proteins were measured by Western blotting analysis. RESULTS: (1) UGT1A mRNA expression was found to be significantly down-regulated in colorectal cancer tissue as compared with the surrounding healthy tissues (P