Premliminary study of NK-4 inhibition on proliferation of colon cancer cells / 中国普通外科杂志
Chinese Journal of General Surgery
;
(12)2001.
Artigo
em Chinês
| WPRIM
| ID: wpr-525995
ABSTRACT
Objective To investigate the influence of selective HGF/cMet inhibitor-NK-4 against colon(cancer) and reveal the potential signaling pathway mechanism of NK-4 effect on colon cancer cell.Methods LoVo colon cancer cells were treated with NK-4(a selective inhibitor of c-Met phosphorylation)at different times.MTT assay and flow cytometry were used to measure cell proliferation and apoptosis.The expression of c-Met,p-c-Met,MEK2,p-ERK and C-myc were measured by Western blot.Results In NK-4-treated group, cells proliferation were inhibited and apoptosis induced in a dose-dependent manner,and resulted in(significant) downregulation of p-c-Met and MEK2/ERK pathway-related protein.The effect of HGF/on LoVo was the opposite.The ratios of p-c-Met,MEK2,p-ERK and C-myc expression between blank group and the NK-4(1?g/mL)-treated for 24h group were 2.58,1.89,1.67 and 2.21(P
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of General Surgery
Ano de publicação:
2001
Tipo de documento:
Artigo
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