Effect of achyranthes bidentata polysaccharides on induction and differentiation of CD4~+T cells / 中国病理生理杂志

ABSTRACT

AIM: To study the immunity modulation mechanism of achyranthes bidentata polysaccharides (ABPS). METHODS: (1) PBMC from patient with asthma or lung cancer were cultured with 800 mg/L ABPS for 18 h. IFN-? and IL-4 mRNA was detected by RT -PCR. (2) Th cells were cultured with serially diluted ABPS for 48 h and with 800 mg/L ABPS for various times. Cultured supernatant was harvested at diffevent time points and RNA was extracted, respectively. IFN-? or IL-4 transcriptional and translational levels were assay by RT -PCR and ELISA. RESULTS: (1) Positive rate of IFN-? mRNA expression in PBMC from patient with asthma or lung cancer increased from 6/25 to 14/25 (P