Age-Related Changes of Glutamate Transporters in the Rat Cerebellum / 체질인류학회지
Korean Journal of Physical Anthropology
;
: 371-380, 2008.
Artigo
em Coreano
| WPRIM
| ID: wpr-52699
ABSTRACT
In the present study, we examined the distribution and amount of two important glutamate transporters, GLT-1 and GLAST in the cerebellum of young and aged rats. Sprague-Dawley rats were used at the age of three months for young control (n=3) and 24 months for aged group (n=4). After transcardial perfusion with 4% paraformaldehyde, brain sections were immunostained for GLT-1, and GLAST. We found that GLT-1- and GLAST-immunoreactive materials were diffusely distributed throughout the gray matter of the cerebellum. Pre-embedding immunoelectron microscopic study demonstrated that the two glutamate transporters in the cerebellum were restricted to glial cells with astrocytic features. The intensity of GLT-1-immunostaining in the cerebellum appeared to be higher in aged rats than in young rats whereas GLAST-immunostaining decreased with aging. Western blot results were also consistent with the immunohistochemical observations. Conclusively, GLT-1 and GLAST expression in the rat cerebellum was changed with aging, i.e, increase of GLT-1 and decrease of GLAST expression with aging, which suggests that the two glutamate transporters might be regulated by different underlying mechanisms with aging.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Perfusão
/
Polímeros
/
Encéfalo
/
Envelhecimento
/
Cerebelo
/
Neuroglia
/
Western Blotting
/
Ratos Sprague-Dawley
/
Ácido Glutâmico
/
Formaldeído
Limite:
Animais
/
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Physical Anthropology
Ano de publicação:
2008
Tipo de documento:
Artigo
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