Trichostatin A, a Histone Deacetylase Inhibitor, Potentiated Cytotoxic Effect of Ionizing Radiation in Human Head and Neck Cancer Cell Lines / 대한방사선종양학회지
The Journal of the Korean Society for Therapeutic Radiology and Oncology
;
: 138-144, 2004.
Artigo
em Inglês
| WPRIM
| ID: wpr-52746
ABSTRACT
PURPOSE:
We have previously reported that human glioblastoma cells are sensitized to radiation-induced death after their exposure to trichostatin A (TSA), a histone deacetylase inhibitor (HDAC-I), prior to the irradiation. We aimed to measure the magnitude of the radiosensitizing effect of TSA in human head and neck cancer cell lines. MATERIALS AND MEHTODS Human head and neck cancer cell lines, HN-3 and HN-9, were exposed to 0, 50, 100, and 200 nM TSA for 18 hr prior to irradiation. Then, the TSA-treated cells were irradiated with 0, 2, 4, 6, and 8 Gy, and cell survival was measured by clonogenic assay.RESULTS:
Pre-irradiation exposure to TSA was found to radiosensitize HN-3 and HN-9 cell lines. In HN-9 cells, the fraction surviving after 2 Gy (SF2) was significantly reduced by treatment of TSA at concentration as low as 50 nM. However, a treatment with 200 nM TSA was required to significantly decrease SF2 in the HN-3 cell line. SER of pre-irradiation treatment with 200 nM TSA was 1.84 in HN-3 and 7.24 in HN-9, respectively.CONCLUSIONS:
Our results clearly showed that human head and neck cancer cell lines can be sensitized to ionizing radiation by pre-irradiation inhibition of histone deacetylase (HDAC) using TSA, and that this potentiation might well be a general phenomenon.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Radiação Ionizante
/
Radiossensibilizantes
/
Histonas
/
Linhagem Celular
/
Sobrevivência Celular
/
Glioblastoma
/
Inibidores de Histona Desacetilases
/
Cabeça
/
Neoplasias de Cabeça e Pescoço
/
Histona Desacetilases
Limite:
Humanos
Idioma:
Inglês
Revista:
The Journal of the Korean Society for Therapeutic Radiology and Oncology
Ano de publicação:
2004
Tipo de documento:
Artigo
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