Effect of BQ123 on the expression of voltage-gated K~+ channel of rat pulmonary artery smooth muscle cells / 中国病理生理杂志

ABSTRACT

AIM: To elucidate the effect of ET-1 on the expression of voltage-gated K~+ channel ? subunits Kv2.1, Kv9.3 in pulmonary artery smooth muscle cells in rats. METHODS: The mRNA expression of Kv2.1, Kv9.3 in the 2nd, 3rd, 4th subculture of intrapulmonary artery smooth muscle cells isolated from Wistar rats, which exposed to either normoxia or chronic hypoxia, were detected with reverse transcription-PCR (RT-PCR). At the same time, PASMCs were treated with BQ123, an ET_A receptor antagonist. RESULTS: The expression of Kv2.1 and Kv9.3 gene were found in the subculture PASMCs of rats exposed either to normoxia or chronic hypoxia. Chronic hypoxia decreased mRNA expressions of Kv channel subunit Kv2.1, Kv9.3 in PASMCs from 0.827?0.126 and 0.388?0.026 to 0.378?0.015 and 0.184?0.009, respectively (P