Simultaneous Assaying of Quercitrin and Isoquercetin in Hypericum Japonicum Thunb.Injection by HPLC / 中国药房
China Pharmacy
;
(12)2001.
Artigo
em Chinês
| WPRIM
| ID: wpr-528020
ABSTRACT
OBJECTIVE:
To establish a HPLC method for the simultaneous determination of quercitrin and isoquercetin in hypericum japonicum thunb.injection.METHODS:
Merck Lichrospher RP 18 was used as chromatographic column and the mo-bile phase consisted of acetonitrile-(pH=3.0)buffer phosphate(18∶82)with flow rate at1ml/min,column temperature at25℃and detection wavelength at350nm.RESULTS:
The linear range for quercitrin and isoquercetin were0.278?g~4.440?g(r=0.9991)and0.315?g~2.520?g(r=0.9991),respectively.The average recovery were98.60%(RSD=2.23%)and97.79%(RSD=1.74%),respectively.CONCLUSION:
This method is accurate,reliable and applicable,and suitable for the quality control of hypericum japonicum thunb.injection.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
2001
Tipo de documento:
Artigo
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