Effect of gene transfer of antisense hypoxia inducible factor-1? on chemosensitivity of human pancreatic cancer cell line BxPC-3 / 中国普通外科杂志
Chinese Journal of General Surgery
; (12)1997.
Article
em Zh
| WPRIM
| ID: wpr-528113
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WPRO
ABSTRACT
Objective To observe the effect of antisense hypoxia inducible factor-1?(HIF-1?) on (chemosensitivity) of human pancreatic cancer cell line BxPC-3 under hypoxia. Methods BxPC-3 cells were divided into 3 groups:(1)BxPC-3 cells were non-transfected with antisense HIF-1? plasmid and exposed to 0.5% O_2 for 4hr(hypoxia control);(2)normoxic BxPC-3 cells were non-transfected with antisense(HIF-1?) plasmid(normoxia control);(3)BxPC-3 cells were transfected with antisense HIF-1? plasmid and exposed to 0.5% O_2 for 4hr(experimental group).Expression of HIF-1? and survivin was detected by RT-PCR and Western Blot.Growth inhibition rates and apoptosis rates of BxPC-3 cells under different(dosages) of chemotherapeutic agents(5-fluorouracil,doxorubicin and gemcitabine) were measured by MTT(colorimetric) assay and flow cytometry (FCM).Results Expression of HIF-1? was obviously down-regulated and at the same time susvivin expression was markedly down-regulated in experimental group(P
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Índice:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of General Surgery
Ano de publicação:
1997
Tipo de documento:
Article