Effect of sonic hedgehog signal pathway blocking on the proliferation and apoptosis of pancreatic cancer cell line SUIT-2 / 中国普通外科杂志
Chinese Journal of General Surgery
; (12)1997.
Article
em Zh
| WPRIM
| ID: wpr-528115
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WPRO
ABSTRACT
Objective To investigate the effect and mechanism of cyclopamine,the inhibitor of Sonic hedgehog signaling pathway,on the proliferation and apoptosis of pancreatic cancer cell line SUIT-2.Methods The SUIT-2 cells used in the experiment were cultured in vitro,and the MTT assay was used to examine the(antiproliferative) effect of cyclopamine.Flow cytometry(FCM) was used to examine the effects of cyclopamine on the proliferation index(PI) and apoptosis index(AI) of SUIT-2 cells.Nude mice tumor graft model was used to determine the effect of cyclopamine on growth inhibition of tumor graft.Results Cyclopamine(produced) time and concentration dependent antiproliferative effects on SUIT-2 cells.Cyclopamine could induce apoptosis and block cell cycle at G_0/G_1 phase.In cyclopamin treated SUIT-2 cells,the apoptosis index(AI) was 14.3?0.35,and proliferation index(PI) was 36.1?0.44;and in control group,the AI was 1.3?0.24,the PI was 52.3?0.28(all P
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Índice:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of General Surgery
Ano de publicação:
1997
Tipo de documento:
Article