Effect of integrin ?2 on adhesion of neuroblastoma cells to collagen / 中国病理生理杂志

ABSTRACT

AIM: To study the effect of integrin ?2 on adhesion of SK-N-SH neuroblastoma cells to collagen. METHODS: Adhesion of the SK-N-SH cells to immobilized collagen was tested with various concentration of Mg~ 2+ , Ca~ 2+ and with 10 ?g/L anti-?2 monoclonal antibody (mAb) 6F1. A_ 570 was detected as adhesion cell numbers. RESULTS: Mg~ 2+ -dependent adhesion of SK-N-SH cells to type I collagen was increased significantly, with peak adhesion at concentration of 1 mmol/L Mg~ 2+ . A_ 570 with or without Mg~ 2+ was 0.59?0.03 and 0.25?0.01 respectively (P