Effect of yigu capsule drug-containing serum on proliferation, ALP activity and expression of interleukin-11 mRNA in rat osteoblasts in the co-culture / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)1989.
Article
em Zh
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| ID: wpr-528134
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WPRO
ABSTRACT
AIM: To investigate the effect of yigu capsule drug-containing serum on proliferation, ALP activity and expression of interleukin-11 mRNA in Sprague-Dawley rat osteobalsts in the co-culture system. METHODS: (1) Osteoblasts and osteoclasts were isolated from 1 and 5-day-old Sprague-Dawley rats, respectively. The osteoblasts-osteoclasts co-culture system was built to prevent the two kinds of cells from contact and allow the media to exchange. The experiment included two groups, drug-containing sera group and control group. (2) The osteoblasts proliferation, ALP activity and expression of interleukin-11 mRNA were detected by the MTT, 4-aminoantipyrine spectrometric methods and FQ-PCR,respectively. RESULTS: In drug-containing sera group, the osteoblasts proliferation, ALP activity, expression of IL-11 mRNA were higher than those of control group (P
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Índice:
WPRIM
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Zh
Revista:
Chinese Journal of Pathophysiology
Ano de publicação:
1989
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Article