Construction of human prostate-specific membrane antigen expression vector and identification of tissue specificity / 中国病理生理杂志

ABSTRACT

AIM:To construct a prostate-specific expression vector of promoter and enhancer of human prostate-specific membrane antigen(PSMA).METHODS:The promoter and enhancer of PSMA were amplified by PCR separately.The two segments were cloned into the expression vector pGL3-Basic,a prostate-specific expression vector pGL3-PSMP-PSME was constructed.The vector was transfected into prostatic carcinoma cell PC-3M and four kinds of non-prostatic carcinoma cells by lipofectamine.The activity of luciferase of transfected cells and tissue specificity of vector was examined at 48 hours after transfection.RESULTS:With DNA sequence of the vector pGL3-PSMP-PSME,the segment of clone was proved correct.The activity of luciferase of the pGL3-PSMP-PSME was expressed distinctly in PC-3M and was not expressed in other nonprostate cell lines.CONCLUSION:The prostate-specific expression vector was constructed successfully.It lays foundation for studying target gene therapy of the prostate cancer.