Determination of Diosgenin in Di’ ao Xinxuekang Capsule by HPLC / 中国药房

ABSTRACT

OBJECTIVE: To establish the method for the determination of diosgenin in Di’ ao Xinxuekang Capsule by HP_LC. METHOD: The condition of HPLC was Shim-pack CLC-Sil columm ( 150mm? 6. 0mm) , mobile phase of 2. 0% isopropand in petroleum ether and UV detector at 206nm. RESULTS: The method proved to be linear in the range at 0. 58～ 11. 6? g of diosgenin with correlation coefficient of 0. 999 8. The linear formula was Y=3. 682? 107X+ 1700, The average recovery and recision were 99. 49% , and RSD=0. 12% ( n=5) . CONCLUSION: The method is simple, accurate, specific and can be used to the quality control of the preparation.