Determination of Midazolam in Children' s Plasma by Solid-phase Extraction-HPLC / 中国药房
China Pharmacy
;
(12)2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-529239
ABSTRACT
OBJECTIVE:
To develop a method for the determination of midazolam in child plasma.METHODS:
Plasma level of midazolam in twenty two ICU children with mechanical ventilation was detected by solid-phase extraction-HPLC after treatment with midazolam for 24h.The separation was performed on Zorbax Eclipse XDB C18 column with acetonitrile — 7.56mmol? L-1 ammonium sulfate(gradient elution) as mobile phase.The flow rate was 1.0mL? min-1,the column temperature was 25℃ and the wavelength of UV detector was 254nm.RESULTS:
The calibration curves for midazolam had a good linearity over the range of 0.05~ 25.00mg? L-1(r=0.999 8).The average recovery for midazolam was 99.29%.Both the intra-day RSD and inter-day RSD were less than 4%.The limits of quantitation for midazolam was 0.01mg? L-1.CONCLUS-IONThe method is sensitive,accurate,simple and rapid,and applicable for clinical blood drug level monitoring of midazolam.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
2005
Tipo de documento:
Artigo
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