Determination of Epirubicin in Human Plasma by RP-HPLC / 中国药房
China Pharmacy
;
(12)1991.
Artigo
em Chinês
| WPRIM
| ID: wpr-530070
ABSTRACT
OBJECTIVE:
To determine the content of epirubicin in human plasma by RP-HPLC.METHODS:
The chromatographic separation was performed on YWG C18 column.The mobile phase consisted of water-acetonitrile(12∶7,with pH adjusted to 3.90?0.05 using 85% phosophoric acid)at a flow rate of 1.0 mL?min-1.The detective wavelength was set at 254 nm.RESULTS:
The linear range of epirubicin was 0.12~4.80 mg?L-1(r=0.998 4)with the lowest detective range of 0.01 mg?L-1.The intraday RSD was less than 4.40% and the inter-day RSD was less than 5.60%,and the average recovery rate was 99.40%(RSD=4.02%).The sample solution was stable within 12 h under room temperature.CONCLUSION:
The method is easy,precise and reproducible to operate and the result of the determination is accurate.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
1991
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS