Optimization of the Extraction and Purification Techniques of Total Flavonoids in Mappianthus Iodoies by Orthogonal Experiment / 中国药房

ABSTRACT

OBJECTIVE:To optimize the extraction and purification techniques of total Flavonoids in Mappianthus iodoies.METHODS: The content of total Flavonoids was determined by UV spectrophotometry. Orthogonal experiment was carried out to investigate the effects of concentration of solvent, the ratio material to liquid, time length of extraction, and frequency of extraction on extraction results of total Flavonoids from Mappianthus iodoies. Under this condition, by means of D-101 macroporous resin, the crude Flavonoids extraction was purified. RESULTS: The best extraction conditions were as follows using 65% (V/V) ethanol as extractor with the ratio of material to liquid at 110,and extracting for 2.5 h/time for 3 times. The content of total Flavonoids in the refined product was above 86%. CONCLUSION: This study provided scientific basis for further research, development and utilization of Mappianthus iodoies.