The effects of ethyl pyruvate on high mobility group box-1 protein expression in murine severe acute pancreatitis / 中国普通外科杂志

ABSTRACT

Objective To explore the effects of ethyl pyruvate (EP) on high mobility group box-1 protein (HMGB1) expression in severe acute pancreatitis (SAP) rats.Methods Ninety male wistar rats were divided randomly into three groups Group A (SAP group); group B (SAP rats received ethyl pyruvate therapy); group C (control group). Specimens from rats in the three groups were taken at 3, 6, 12, 24 and 48 h after operation respectively. The concentration of plasma amylase and D-lactate the activity of malonyl dialdehyde (MAD) in the intestinal tissue were determined. The changes of morphological damage of intestinal tissue was observed by microscopy. The expression of HMGB1 in intestinal mucosa was observed by SP immunohistochemistry and the activity of HMGB1 was determined by western blot.Results Compared with group A, Ievels of plasma amylase, and D-lactate in group B decreased markedly (P