Determination of Drug Loading Rate of Butyl-5-Aminosalicylic Acid-Pectin Conjugates by HPLC / 中国药房
China Pharmacy
;
(12)2001.
Artigo
em Chinês
| WPRIM
| ID: wpr-530288
ABSTRACT
OBJECTIVE:
To establish an HPLC method for the determination of the drug loading rate of butyl-5-aminosalicylic acid-pectin conjugate.METHODS:
The conjugate was hydrolyzed with base solution and neutralized with acid before being determined by HPLC.Kromasil C18 column was used as chromatographic column with mobile phase consisted of water(containing 0.15% triethylamine and 0.15% acetic acid)-methanol(95∶5,V/V).The detection wavelength was set at 240 nm.RESULTS:
The calibration curve of 5-aminosalicylic acid was linear over the range of 1~100 ?g?mL-1(r=0.999 9) at an average recovery of 99.90%.Both intra-day and inter-day RSD were less than 2.25%.The drug loading rate of the conjugate was 12.76 %(n=3).CONCLUSION:
The method is simple,rapid,accurate,and suitable for the determination of drug-loading rate of conjugate.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
2001
Tipo de documento:
Artigo
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