Determination of Total Flavonoids in Extract of Bidens bipinnata L. by Spectrophotometry / 中国药房

ABSTRACT

OBJECTIVE: To determine the content of total flavonoids in extract of Bidens bipinnata L. by spectrophotometry. METHODS: The Bidens bipinnata L. was extracted with ethanol and purified by HPD100 macroporous resin. With rutin as reference substance, the content of total flavonoids in the extract of Bidens bipinnata L. was determined by spectrop-hotometry at a wavelength of 510 mn. RESULTS: The linear range of Rutin was 0.008 16～0.048 96 mg?mL-1(r=0.999 3), its average recovery was 99.03% (RSD=2.27%,n=6).CONCLUSION: The method is of high sensitivity and good stability, and it is applicable for the quality control of Bidens bipinnata L.