Determination of Gastrodin in Shulenaoxintong Granules by HPLC / 中国药房

ABSTRACT

OBJECTIVE: To establish an HPLC method for the determination of Gastrodine in Shulenaoxintong granules. METHODS: The chromatographic column was Hypersil ODS(250 mm?4.0 mm,5 ?m) with a mobile phase consisted of acetonitrile-0.05%phosphorin acid(2∶98) at a flow rate of 1 mL?min-1. The detective wavelength was set at 220 nm. RESULTS: A good linearity was obtained in the range of 0.1～2.0 ?g(r=1.000 0), and the average recovery was 96.6% (RSD=0.80%,n=6). CONCLUSION: The method is convenient, rapid, and reproducible, and it is applicable for the qua-lity control of Shulenaoxintong granules.