Reversal of multidrug resistance by transfection of tumor necrosis factor ? and MDR1 antisense RNA into multidrug resistant breast cancer cell line / 中国病理生理杂志

ABSTRACT

AIM:To study the reversal effects of multidrug resistance by transfecting tumor necrosis factor ?(TNF-?) cDNA and multidrug resistant 1(MDR1) gene antisense RNA into multidrug resistant breast cancer cell line MCF-7/ADR.METHODS:The recombinant vector of enhanced green fluorescent protein(EGFP) with MDR1 antisense RNA and recombinant vector of red fluorescent protein(DsRed2) with TNF-? cDNA were constructed by RT-PCR and DNA recombinant techniques.The recombinant vectors were transfected into multidrug resistant breast cancer cell line MCF-7/ADR.The cell growth curves,cell apoptosis rates,MDR1 gene expression at mRNA and P-gp levels,and the sensitivity to ADR were determined before and after the transfection.RESULTS:After the transfection,cells showed lower growth rate,higher apoptosis rate,lower MDR1 expression at mRNA and P-gp levels,and the sensitivity to ADR increased significantly.CONCLUSION:Transfection of TNF-? cDNA and MDR1 antisense RNA into multidrug resistant breast cancer cells may have good effects on reversal of multidrug resistance.