Effect of LY294002 on Cell Cycle and Apoptosis of Multidrug Resistant HL60/VCR Cell Line / 中国药房
China Pharmacy
;
(12)2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-530917
ABSTRACT
OBJECTIVE:
To explore the effect of the specific inhibitor-LY294002 of the signaling pathway PI3K/AKT on cell cycle and apoptosis of HL60/VCR cells and its mechanism.METHODS:
Vincristine(VCR) resistant HL60/VCR were treated with LY294002(final concentration at 1.0,2.5,5.0,or 10 ?mol?L-1) or without LY294002(control).IC50 was detected by MTT;flow cytometry was used to evaluate apoptosis and cell cycle distribution,and changes of Bcl-2,CyclinD1 gene mRNA levels were detected by reverse transcription polymerase chain reaction.RESULTS:
As compared with control,LY294002 significantly reduced the IC50 value in HL60/VCR to vincristine in a concentration dependent manner(P
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
2005
Tipo de documento:
Artigo
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