Reversal of Multidrug Resistance in NPC HNE-1(200) Cell Line by Oxymatrine / 中国药房
China Pharmacy
;
(12)2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-531068
ABSTRACT
OBJECTIVE:
To study the reversal mechanism of oxymatrine on multidrug resistance in the NPC HNE-1(200) cell line.METHODS:
The multidrug resistant HNE-1(200) subline was derived from an HNE-1 cell line after exposure to fractionated X-irradiation. The expression of P-gp was tested by immunocytochemical method and western blot and that of mdr1 mRNA was measured by RT-PCR.RESULTS:
After 24-hour treatment with oxymatrine, both the immunocytochemical method and western blot showed a decrease in the expression of P-gp. However, RT-PCR measurement showed that the expression of mdr1 mRNA of HNE-1 and HNE-1(200) was nonsignficant.CONCLUSION:
Oxymatrine can reverse the multidrug resistance of NPC HNE-1(200) cell line to some degree by down-regulating the expression of P-gp. However, this medicine did no effect on the expression of mdr1 mRNA, suggesting the up-regulation of the expression of P-gp is under the control of multiple mechanism.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
2005
Tipo de documento:
Artigo
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