Detemination of Tobramycin in Tobramycin Dexamethasone Eye Drops by HPLC-ELSD / 中国药房
China Pharmacy
;
(12)2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-531081
ABSTRACT
OBJECTIVE:
To determine the content of tobramycin in Tobramycin Dexamethasone Eye Drops by HPLC-ELSD.METHODS:
The sample was separated on VP-ODS C18 column.The mobile phase was 1.0% trifluoroacetic acid-methanol(95∶5) at a flow rate of 1.0 mL?min-1.The drift tube temprature was 45 ℃;the pressure of nebulizing gas was 3.5 bar,and the detection wavelength was set at 254 nm.RESULTS:
The liner range of tobramycin was 156.0~436.0 ?g?mL-1(r=0.999 7).The limit of detection was 0.523 ?g and the limit of quantification was 1.744 ?g.The average recovery was 99.57%(RSD=0.92%).CONCLUSION:
The method is accurate,reliable,specific,and suitable for the quality control of Tobramycin Dexamethasone Eye Drops.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
2007
Tipo de documento:
Artigo
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