Improvement in HPLC for Determination of Serum Concentration of Isofosfamide / 中国药房
China Pharmacy
;
(12)2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-531144
ABSTRACT
OBJECTIVE:
To establish a simple and rapid HPLC method for the determination of serum concentration of isofosfamide.METHODS:
With cyclophosphamide as internal standard,the serum sample was extracted on C18 solid phase extraction column and determined on Zorbox-ODS C18 at room temperature.The mobile phase consisted of acetonitrile-water(25∶75) at a flow rate of 1 mL?min-1 and a detection wavelength of 200 nm.RESULTS:
The linear range of ifosfamide was 2.5~200 ?g?mL-1(r=0.999 9),with the lowest detectable limit at 0.5 ?g?mL-1(S/N=3).Both the intraday and interday RSD were less than 5%.The extraction recoveries of ifosfamide at high,middle and low concentrations were ranged between 89.3% and 93.6% versus 77.6%~82.3% in chloroform-treated serum samples.CONCLUSION:
The method which uses the C18 solid phase extraction column is of high recovery,and the sample treatment by which is simple,rapid,accurate,and pollution-free,and it is applicable for the monitoring of serum concentration and pharmacokinetic study of isofosfamide.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
2007
Tipo de documento:
Artigo
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