Determination of Serum Concentration of Phenobarbital by RP-HPLC / 中国药房
China Pharmacy
;
(12)2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-531146
ABSTRACT
OBJECTIVE:
To determine the concentration of phenobarbital(PBB) in serum by RP-HPLC.METHODS:
The analytical column was C18.The mobile phase consisted of methanol-water(44∶56) at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 205 nm and the temperature of column was set at 35℃.RESULTS:
A good linear relationship was obtained for PBB at a concentration range from 5.08 ?g?mL-1 to 63.50 ?g?mL-1(r=0.999 9).The mean relative recovery was 99.75% and the mean extraction recovery was 96.34%.The intra-day RSD and the inter-day RSD were all less than 4%.CONCLUSION:
This method is simple,rapid,accurate,and suitable for therapeutic drug monitoring.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
2007
Tipo de documento:
Artigo
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