Determination of Phenylhydrazine Residues in Edaravone by HPLC / 中国药房

ABSTRACT

OBJECTIVE: To establish an HPLC method for the determination of phenylhydrazine residues in edaravone.METHODS: The determination was performed on Diamonsil C18 column with 0.05 mol?L-1 ammonium acetate-acetonitrile(80∶20) as mobile phase at a flow of 1.0 mL?min-1.The wavelength of UV detector was set at 233 nm and the sample size was 20 ?L.RESULTS: The linear range of phenylhydrazine was 22～550 ng?mL-1(r=0.999 9);The average recovery was 98.10%(RSD=0.85%),and the detection limit was 44 pg.CONCLUSION: The analytical method is simple,reliable and sensitive,and suitable for the determination of phenylhydrazine residues in edaravone preparation.