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Construction of fusion expression vector AR-GFP and its expression in Hek293 cells / 中国病理生理杂志
Chinese Journal of Pathophysiology ; (12)2000.
Artigo em Chinês | WPRIM | ID: wpr-531410
ABSTRACT

AIM:

To construct a hAR and GFP fusion gene vector and to observe the AR-GFP gene expression in Hek293 cells.

METHODS:

A recombined vector pcDNA3.1/myc-HisA-AR-GFP (pH-AG) was constructed by gene engineering technique. The recombined vector was transfected into Hek293 cells using calcium phosphate.

RESULTS:

AR-GFP fusion protein was successfully expressed in Hek293 cells without biologic activity, which was confirmed by fluorescence microscopy and Western blotting.

CONCLUSION:

The Hek293 cells transfected by AR-GFP fusion gene can express its protein successfully. However, it is not a cellular model for ARI screening.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Journal of Pathophysiology Ano de publicação: 2000 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Journal of Pathophysiology Ano de publicação: 2000 Tipo de documento: Artigo