Construction of fusion expression vector AR-GFP and its expression in Hek293 cells / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)2000.
Artigo
em Chinês
| WPRIM
| ID: wpr-531410
ABSTRACT
AIM:
To construct a hAR and GFP fusion gene vector and to observe the AR-GFP gene expression in Hek293 cells.METHODS:
A recombined vector pcDNA3.1/myc-HisA-AR-GFP (pH-AG) was constructed by gene engineering technique. The recombined vector was transfected into Hek293 cells using calcium phosphate.RESULTS:
AR-GFP fusion protein was successfully expressed in Hek293 cells without biologic activity, which was confirmed by fluorescence microscopy and Western blotting.CONCLUSION:
The Hek293 cells transfected by AR-GFP fusion gene can express its protein successfully. However, it is not a cellular model for ARI screening.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Pathophysiology
Ano de publicação:
2000
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS