Simultaneous Determination of Berberin Hydrochloride,Baicalin and Glibenclamide in Kangtang Tablets by RP-HPLC / 中国药房
China Pharmacy
;
(12)2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-531460
ABSTRACT
OBJECTIVE:
To establish an RP-HPLC method for simultaneous determination of Berberin hydrochloride,Baicalin and Glibenclamide in Kangtang tablets.METHODS:
Separation of samples was achieved on a Diamond C18 analytical column(250 mm?4.6 mm,5 ?m).The mobile phase consisted of methanol-0.3% H3PO4(containing 0.3% triethylamine,pH2.5)using gradient program at a flow rate of 1.0 mL?min-1 and a detection wavelength of 230 nm.RESULTS:
The linear ranges of Berberin hydrochloride,Baicalin and Glibenclamide were 0.02~1.0 mg?mL-1,0.02~1.0 mg?mL-1,0.000 8~0.04 mg?mL-1,respectively.The average recoveries were 100.16%,99.32% and 97.23%,respectively.The RSD were 1.97%、1.77%、1.28%,respectively(n=6).CONCLUSION:
The method is simple,sensitive with high recovery rates and it is suitable for the quality control of Kangtang tablets.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
2007
Tipo de documento:
Artigo
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