Involvement of JNK-initiated p53 accumulation and phosphorylation of p53 in pseudolaric acid B induced cell death
Experimental & Molecular Medicine
; : 428-434, 2006.
Article
em En
| WPRIM
| ID: wpr-53147
Biblioteca responsável:
WPRO
ABSTRACT
A terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay was used to determine that apoptosis causes HeLa cell death induced by pseudolaric acid B. The c-Jun N-terminal kinase (JNK) inhibitor SP600125 decreased p53 protein expression during exposure to pseudolaric acid B. SP600125 decreased the phosphorylation of p53 during pseudolaric acid B exposure, indicating that JNK mediates phosphorylation of p53 during the response to pseudolaric acid B. SP600125 reversed pseudolaric acid B-induced down-regulation of phosphorylated extracellular signal-regulated protein kinase (ERK), and protein kinase C (PKC) was activated by pseudolaric acid B, whereas staurosporine, calphostin C, and H7 partly blocked this effect. These results indicate that p53 is partially regulated by JNK in pseudolaric acid B-induced HeLa cell death and that PKC participates in pseudolaric acid B-induced HeLa cell death.
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Texto completo:
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Índice:
WPRIM
Assunto principal:
Fosforilação
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Proteína Quinase C
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Células HeLa
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Proteína Supressora de Tumor p53
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Morte Celular
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Proteínas Quinases JNK Ativadas por Mitógeno
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Diterpenos
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Fragmentação do DNA
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Antracenos
Limite:
Humans
Idioma:
En
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2006
Tipo de documento:
Article