Metabolic loading of guanosine induces chondrocyte apoptosis via the Fas pathway
Experimental & Molecular Medicine
;
: 401-407, 2006.
Artigo
em Inglês
| WPRIM
| ID: wpr-53150
ABSTRACT
Although the apoptosis of chondrocytes plays an important role in endochondral ossification, its mechanism has not been elucidated. In this study, we show that guanosine induces chondrocyte apoptosis based on the results of acridine orange/ ethidium bromide staining, caspase-3 activation, and sub-G1 fraction analysis. The potent inhibitory effect of dipyridamole, a nucleoside transporter blocker, indicates that extracellular guanosine must enter the chondrocytes to induce apoptosis. We found that guanosine promotes Fas-Fas ligand interaction which, in turn, leads to chondrocyte apoptosis. These findings indicate a novel mechanism for endochondral ossification via metabolic regulation.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Glicoproteínas de Membrana
/
Transdução de Sinais
/
Ratos Sprague-Dawley
/
Apoptose
/
Receptores do Fator de Necrose Tumoral
/
Receptor fas
/
Condrócitos
/
Proteínas de Transporte de Nucleosídeos
/
Fatores de Necrose Tumoral
/
Proteína Ligante Fas
Limite:
Animais
Idioma:
Inglês
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2006
Tipo de documento:
Artigo
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