Effect of recombinant MIF on lung fibroblast proliferation and collagen synthesis in vitro / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1986.
Artigo
em Chinês
| WPRIM
| ID: wpr-532146
ABSTRACT
AIM:
To investigate the influence and mechanism of recombinant macrophage migration inhibitory factor(rMIF) on fibroblasts.METHODS:
MRC-5 fibroblasts were divided into two groups the treated group was treated with rMIF(25-100 ?g/L,12 h,24 h or 48 h) and the control was non-rMIF treatment.The activity of proliferation in both groups was investigated and compared by CCK-8 means.Synthesis of collagen in the culture supernatants was detected by the hydroxyproline.The expression of collagen type I mRNA was examined using RT-PCR analysis.The level of collagen type I protein induced by rMIF was quantified by Western blotting.RESULTS:
The production of proliferation ratio of fibroblasts treated with 50 ?g/L and 100 ?g/L rMIF at 24 h or 48 h were increased obviously(P
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Pathophysiology
Ano de publicação:
1986
Tipo de documento:
Artigo
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