Determination of the Concentration of New Antithrombotic Medicine Dermatan Sulfate in Blood or Urine by Spectrophotometry / 中国药房

ABSTRACT

OBJECTIVE:To determine the concentration of Dermatan Sulfate,a new antithrombotic medicine in blood or urine by spectrophotometry.METHODS:In Britton Robinson buffer solution(pH=5.8),color fading reaction occurred when Dermatan sulfate combined with neutral red dye to form ionic associate.The reduction of absorbance of the system was positively correlated to dermatan sulfate concentration,with the maximum absorption wavelength at 526 nm.RESULTS:The linear range of dermatan sulfate was 0.18～4.0?g?mL~(-1)(r=0.999 1) with a detection limit of 0.054?g?m~(-1).The average recovery was 100.3%(RSD=1.2%),with RSD of dermatan sulfate at low,medium,and high concentrations at 2.9%, 1.5%,and 1.1%,respectively.The coexisting substances did no interference on the determination results.CONCLUSION: The method is simple,accurate,and sensitive with good methodological selectivity and it achieved satisfactory results in the determination of dermatan sulfate in blood or urine.