Inhibitory Effect of Arsenic Trioxide on Human Gastric Cancer Cell SGC-7901 under Hypoxia Condition / 中国药房

ABSTRACT

OBJECTIVE:To investigate the effect of arsenic trioxide(As2O3) on the growth inhibition and apoptosis of gastric cancer cell SGC-7901 cultured in vitro in hypoxia condition.METHODS:The environment of hypoxia was established by using cobalt chloride.The gastric cancer cells were divided into three groupsnormal group,hypoxia group and hypoxia combined with arsenic trioxide(0.5,1.0,2.0,5.0 and 10.0 ?mol?L-1)(HAT) group.The optical density of gastric cancer cell was determined by MTT method and inhibition rate was calculated at 24,48 and 72 h.The apoptosis rate of gastric caner cell was detected by using staining technique.RESULTS:After treated by 10.0 ?mol?L-1 arsenic trioxide for 24,48 and 72 h,the inhibition rates of SGC-7901 were 31.99%,55.98% and 72.40% for HAT group and 4.04%,6.65% and 8.11% for hypoxia groups,respectively;apoptosis rate were(42.88?1.72)%,(56.48?1.48)% and(65.52?1.00)% for HAT group and(2.08?0.28)%,(2.44?0.51)% and(2.76?0.67)% for hypoxia group,respectively.The difference between HAT group and hypoxia group was statistically significant(P